Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Breast
Cell type
Mammary glands
NA
NA

Attributes by original data submitter

Sample

source_name
Mammary gland
strain
Mixed C57BL/6 x FVB/N
tissue
Mammary gland
state
pregnancy day of 13
genotype
wild type
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Frozen-stored mammary tissues from p13 were broken into powder with mortar and pestle. Nuclei were extracted in Farnham lysis buffer (5mM PIPES PH8.0, 85mM KCl, 0.5% NP-40 and protease inhibitor). Antibodies against EZH1 (gift from Sartorelli, Mousavi et al. Mol. Cell 2012), EZH2 (Diagenode, cat# C15410039 and lot# 003), STAT5 (Santa Cruz Biotechnology, cat# sc-1081; L-20, lot# L2012, 10ug) H3K27me3 (Millipore, 07-449) and PolII (Abcam, cat# ab5408, lot# GR106949-6) were used for ChIP. Libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
31307324
Reads aligned (%)
99.0
Duplicates removed (%)
12.5
Number of peaks
562 (qval < 1E-05)

mm9

Number of total reads
31307324
Reads aligned (%)
98.9
Duplicates removed (%)
12.6
Number of peaks
582 (qval < 1E-05)

Base call quality data from DBCLS SRA