Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Larvae
Cell type
L4
NA
NA

Attributes by original data submitter

Sample

source_name
Whole animal
developmental stage
L4 larva
strain
pJK1662

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Immunocomplexes, from each sample, were collected using 10 µg of M2 Flag antibody (F1804 Sigma) or 10 µg of 9E10 Myc antibody (Sigma M4439) overnight at 4 °C. Then each sample was incubated for 1-2hr at 4 °C with 75 µL Dynabeads-Protein G (Novex 10003D). Lysate samples and immunoprecipated protein samples were analyzed by immunoblot analysis using standard protocols. ChIP-Seq DNA libraries were prepared using standard protocols and assessed for integrity using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). DNA sequencing was performed on four lanes of an Illumina HiSeq2000 at the University of Wisconsin-Madison Biotechnology Center (Madison, WI).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

ce11

Number of total reads
17431794
Reads aligned (%)
98.7
Duplicates removed (%)
18.2
Number of peaks
724 (qval < 1E-05)

ce10

Number of total reads
17431794
Reads aligned (%)
98.7
Duplicates removed (%)
18.2
Number of peaks
729 (qval < 1E-05)

Base call quality data from DBCLS SRA