Lysates were clarified from sonicated nuclei and Nr4a1-DNA complexes were isolated with antibody (anti-NR4A1 antibody, clone H1648, cat#PP-H1648-00, lot#A-1, Perseus Proteomics). Libraries were prepared according to Illumina's instructions accompanying the TruSeq ChIP Sample Prep Kit (Cat# IP-202-1012). Briefly, DNA was end-repaired, then protruding 3- 'A' bases were ligated to Illumina's adapters which have a single 'T' base overhang at the 3’ end. After adapter ligation DNA was PCR amplified with Illumina primers for 18 cycles and library fragments of 250~350 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation, using TruSeq SR Cluster Kit vs-cBot-HS (Cat#GD-401-3001). Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols, Using TruSeq SBS Kit v3-HS (Cat#FC-401-3002).