Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Others
Cell type
Mesenchymal stem cells
NA
NA

Attributes by original data submitter

Sample

source_name
Mesenchymal stem cell
genotype/variation
KO
antibody
H3K4me3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody(anti-H3K4me3 (CST, 9751s) and normal rabbit IgG (Santa Cruz, SC-2027)). Libraries were prepared by library construction NEBNext kit according to the manufacturer’s protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
10733659
Reads aligned (%)
98.2
Duplicates removed (%)
2.1
Number of peaks
12543 (qval < 1E-05)

hg19

Number of total reads
10733659
Reads aligned (%)
97.6
Duplicates removed (%)
2.2
Number of peaks
12405 (qval < 1E-05)

Base call quality data from DBCLS SRA