Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MYC

Cell type

Cell type Class
Blood
Cell type
GEN2.2
NA
NA

Attributes by original data submitter

Sample

source_name
GEN2.2 cells
cell line
GEN2.2
antibody
anti-MYC

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
unstimulated GEN2.2 cells were cross linked with 1% of formaldehyde for 10 min at 37°C, sonicated and subjected to immunoprecipitation with 2 μg of anti-MYC antibody (Santacruz Biotechnology, N262X), or rabbit IgG control. After reverse cross-linking and recovering the chromatin, the isolated chromatin was subjected to library preparation using NEXTflex Rapid DNA-Seq Kit (BIOO Scientific) according to manufacturer’s instruction with the following exceptions: 1) a bead size selection was performed before the PCR amplification and 2) the libraries were size-selected between 200-300 bp by 8% PAGE before sequencing.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
26473941
Reads aligned (%)
26.5
Duplicates removed (%)
45.0
Number of peaks
4358 (qval < 1E-05)

hg38

Number of total reads
26473941
Reads aligned (%)
27.2
Duplicates removed (%)
44.0
Number of peaks
4310 (qval < 1E-05)

Base call quality data from DBCLS SRA