GSM1722714: GEN2.2-anti-MYC; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
TFs and others
Cell type Class
Attributes by original data submitter
Sequenced DNA Library
unstimulated GEN2.2 cells were cross linked with 1% of formaldehyde for 10 min at 37°C, sonicated and subjected to immunoprecipitation with 2 μg of anti-MYC antibody (Santacruz Biotechnology, N262X), or rabbit IgG control. After reverse cross-linking and recovering the chromatin, the isolated chromatin was subjected to library preparation using NEXTflex Rapid DNA-Seq Kit (BIOO Scientific) according to manufacturer’s instruction with the following exceptions: 1) a bead size selection was performed before the PCR amplification and 2) the libraries were size-selected between 200-300 bp by 8% PAGE before sequencing.