GSM1722714: GEN2.2-anti-MYC; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
MYC
Cell type
Cell type Class
Blood
Cell type
GEN2.2
NA
NA
Attributes by original data submitter
Sample
source_name
GEN2.2 cells
cell line
GEN2.2
antibody
anti-MYC
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
unstimulated GEN2.2 cells were cross linked with 1% of formaldehyde for 10 min at 37°C, sonicated and subjected to immunoprecipitation with 2 μg of anti-MYC antibody (Santacruz Biotechnology, N262X), or rabbit IgG control. After reverse cross-linking and recovering the chromatin, the isolated chromatin was subjected to library preparation using NEXTflex Rapid DNA-Seq Kit (BIOO Scientific) according to manufacturer’s instruction with the following exceptions: 1) a bead size selection was performed before the PCR amplification and 2) the libraries were size-selected between 200-300 bp by 8% PAGE before sequencing.