Antigen

Antigen Class

TFs and others

Antigen

MYC

Cell type

Cell type Class

Blood

Cell type

GEN2.2

NA

NA

Sample

source_name

GEN2.2 cells

cell line

GEN2.2

antibody

anti-MYC

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

unstimulated GEN2.2 cells were cross linked with 1% of formaldehyde for 10 min at 37°C, sonicated and subjected to immunoprecipitation with 2 μg of anti-MYC antibody (Santacruz Biotechnology, N262X), or rabbit IgG control. After reverse cross-linking and recovering the chromatin, the isolated chromatin was subjected to library preparation using NEXTflex Rapid DNA-Seq Kit (BIOO Scientific) according to manufacturer’s instruction with the following exceptions: 1) a bead size selection was performed before the PCR amplification and 2) the libraries were size-selected between 200-300 bp by 8% PAGE before sequencing.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg19

Number of total reads

26473941

Reads aligned (%)

26.5

Duplicates removed (%)

45.0

Number of peaks

4358 (qval < 1E-05)

hg38

Number of total reads

26473941

Reads aligned (%)

27.2

Duplicates removed (%)

44.0

Number of peaks

4310 (qval < 1E-05)