Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
embryonic stem cells_input
cell line
CGR8
cell type
embryonic stem cells

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from enzyme-digested nuclei and protein-DNA complexes were pulled-down with antibody (anti-Bcl3, catalog number GTX108293, GeneTex) Libraries were prepared according to Illumina's instructions, including end repair, 3' end adneylation, ligation with adaptors and DNA enrichment.

Sequencing Platform

instrument_model
Illumina Genome Analyzer

mm10

Number of total reads
25926962
Reads aligned (%)
96.1
Duplicates removed (%)
14.0
Number of peaks
328 (qval < 1E-05)

mm9

Number of total reads
25926962
Reads aligned (%)
96.0
Duplicates removed (%)
14.0
Number of peaks
306 (qval < 1E-05)

Base call quality data from DBCLS SRA