GSM1717865: MDA-MB453 PIAS1 R1881 rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
PIAS1
Cell type
Cell type Class
Breast
Cell type
MDA-MB-453
Primary Tissue
Breast
Site of Extraction
Effusion, Pericardial
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
MDA-MB453_PIAS1_R1881
origin
Metastatic carcinoma of the breast
cell line
MDA-MB453 breast cancer cell line
treated with
10 nM R1881 for 2hrs
chip antibody
PIAS1 (Abcam 77231)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were derived from sonicated nuclei and AR- and PIAS1-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocol, as utilized at the Functional Genomics Unit, Helsinki, Finland.