Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AR

Cell type

Cell type Class
Breast
Cell type
MDA-MB-453
Primary Tissue
Breast
Site of Extraction
Effusion, Pericardial
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
MDA-MB453_AR_siPIAS1_R1881
origin
Metastatic carcinoma of the breast
cell line
MDA-MB453 breast cancer cell line
transfected with
PIAS1 siRNA
treated with
10 nM R1881 for 2hrs
chip antibody
rabbit antiserum against AR

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were derived from sonicated nuclei and AR- and PIAS1-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocol, as utilized at the Functional Genomics Unit, Helsinki, Finland.

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
39191369
Reads aligned (%)
80.9
Duplicates removed (%)
12.3
Number of peaks
33197 (qval < 1E-05)

hg38

Number of total reads
39191369
Reads aligned (%)
82.3
Duplicates removed (%)
11.4
Number of peaks
33189 (qval < 1E-05)

Base call quality data from DBCLS SRA