Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Neural

Cell type

BE(2)-C

Primary Tissue

Brain

Site of Extraction

Bone Marrow

Tissue Diagnosis

Neuroblastoma

Sample

source_name

neuroblastoma BE(2)-C cells transfected with control siRNA

cell type

peripheral nervous system tumour cell

cell line

BE(2)-C

chip antibody

H3K4me3 (Millipore, Millipore, 07-473, lot JBC1863338)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. DNA immunoprecipitated by the antibodies were purified and subjected to sequencing with Illumina HiSeq 2000 at Ramaciotti Centre for Genomics, University of New South Wales, Australia

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

46736312

Reads aligned (%)

89.8

Duplicates removed (%)

44.2

Number of peaks

24851 (qval < 1E-05)

hg19

Number of total reads

46736312

Reads aligned (%)

89.5

Duplicates removed (%)

44.7

Number of peaks

24787 (qval < 1E-05)