Antigen

Antigen Class

TFs and others

Antigen

Rnf2

Cell type

Cell type Class

Embryo

Cell type

Forelimb

MeSH Description

A front limb of a quadruped. (The Random House College Dictionary, 1980)

Sample

source_name

forelimb buds

strain

CD1

age

E11.5

antibody

RING1B (Activ Motif 39664, Lot. 23012002)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Antibodies against RING1B (Active Motif 39664, Lot. 23012002) was used for immunoprecipitation. Purified immunoprecipitated DNA prepared using the TruSeq ChIP Sample Preparation Kit. Samples were sequenced on the Illumina HiSeq system using 100 bp single-end reads according to the manufacturer’s specifications. Illumina TruSeq protocol

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

43650149

Reads aligned (%)

90.7

Duplicates removed (%)

50.1

Number of peaks

2451 (qval < 1E-05)

mm9

Number of total reads

43650149

Reads aligned (%)

90.5

Duplicates removed (%)

50.2

Number of peaks

2408 (qval < 1E-05)