GSM1716497: HCT116 p53+/+ IgG (Mock); Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
HCT116 p53+/+ cell line
cell line
HCT116
cell type
Human colorectal carcinoma
chip antibody
Normal Rabbit IgG (Santa Cruz Biotechnology, sc-2027)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation (ChIP) was performed using ChIP assay kit (Upstate Biotechnology) following the manufacturer’s instructions. Lysates obtained after sonication were subjected to pull down with antibody. ChIP enriched DNA was subjected to library construction using Illumina's "Preparing samples for ChIP sequencing of DNA" kit. Briefly, DNA ends were repaired and phosphorylated. 3'-dA overhangs were generated using Klenow (exo-) and dATP. Thereafter, the adaptors were ligated. PCR amplification for 18 cycles and size selection was performed.