Antigen

Antigen Class

TFs and others

Antigen

HDAC2

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

hESCs

cell type

human embryonic stem cells (hESCs)

passages

p21

reprogramming method

None

clone

None

chip antibody

HDAC2 (Cell Signaling, 2545S)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

RNA-seq:mRNA was extracted using the miRNEASY kit from Qiagen ChIP-seq:Cells were crosslinked with formalin RNA-seq: Ovation® RNA-Seq System V2 kit (NuGEN) and NEBNext DNA Library Prep Master Mix Set for Illumina ChIP-seq: NEBNext DNA Library Prep Master Mix Set for Illumina or NEBNext® Ultra™ DNA Library Prep

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

15916251

Reads aligned (%)

97.5

Duplicates removed (%)

40.6

Number of peaks

298 (qval < 1E-05)

hg19

Number of total reads

15916251

Reads aligned (%)

97.1

Duplicates removed (%)

40.7

Number of peaks

167 (qval < 1E-05)