Antigen

Antigen Class

Histone

Antigen

H3K4me2

Cell type

Cell type Class

Blood

Cell type

CD4+ T cells

NA

NA

Sample

source_name

Splenic CD4+ T cells

tissue

spleen

cell type

CD4+ T cells

strain

AND

peptide

10uM PCC

timepoint

24 hours

chip antibody

H3K4me2 (Millipore, cat. no. 07-030)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Quoted from GSE62826: "Sequencing libraries were prepared from collected DNA by blunting, A-tailing, adapter ligation as previously described (Heinz, S. et al. Molecular Cell 38, 576-589 (2010)) using barcoded adapters (NextFlex, Bioo Scientific). Libraries were PCR-amplified for 12-15 cycles, size selected by gel extraction and sequenced on a Hi-Seq 2000 (Illumina) for 51 cycles." CD4+ cells were re-isolated from the culture using the Miltenyi MACS system.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

20758028

Reads aligned (%)

98.7

Duplicates removed (%)

9.3

Number of peaks

12526 (qval < 1E-05)

mm9

Number of total reads

20758028

Reads aligned (%)

98.6

Duplicates removed (%)

9.4

Number of peaks

12553 (qval < 1E-05)