GSM1703649: AND CD4TCell H3K4me2 100uM K99A 24h Five Conditions; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me2
Cell type
Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA
Attributes by original data submitter
Sample
source_name
Splenic CD4+ T cells
tissue
spleen
cell type
CD4+ T cells
strain
AND
peptide
100uM K99A
timepoint
24 hours
chip antibody
H3K4me2 (Millipore, cat. no. 07-030)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Quoted from GSE62826: "Sequencing libraries were prepared from collected DNA by blunting, A-tailing, adapter ligation as previously described (Heinz, S. et al. Molecular Cell 38, 576-589 (2010)) using barcoded adapters (NextFlex, Bioo Scientific). Libraries were PCR-amplified for 12-15 cycles, size selected by gel extraction and sequenced on a Hi-Seq 2000 (Illumina) for 51 cycles." CD4+ cells were re-isolated from the culture using the Miltenyi MACS system.