Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Blood

Cell type

Thymus

NA

NA

Sample

source_name

thymocytes

cell type

thymocytes

genotype

n/a

strain

n/a

chip antibody

H3K4me3

age

Mixture: 4 months or 1.5 years

Sex

female

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

cells were crosslinked with 1% HCHO, quenched with 0.125 M glycine, washed and resuspended in RIPA buffer (10 mM Tris pH 7.4, 1 mM EDTA, 1% tritonX-100, 0.1% sodium deoxycholate, 0.1% SDS) containing 0.8 M NaCl and sonicated using a water bath sonicator (Diagenode) to obtain DNA of ≈ 300–500 bp. libraries were prepared and sequenced following standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

20488401

Reads aligned (%)

91.8

Duplicates removed (%)

29.5

Number of peaks

25389 (qval < 1E-05)

hg19

Number of total reads

20488401

Reads aligned (%)

91.6

Duplicates removed (%)

29.7

Number of peaks

25252 (qval < 1E-05)