Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TRIM24

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
LNCaP
cell line
LNCaP
treatment
10nM DHT
chip antibody
TRIM24 (Bethyl, A300-815A)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated cells and chromatin-DNA complexes were isolated with the indicated antibody. Library preparation was done with the ThruPLEX-FD Prep Kit from Rubicon Genomics (R40048), which results in ChIP-seq libraries compatible with Illumina NGS Platforms. The Illumina Hiseq-2000 platform was used for sequencing.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
29405113
Reads aligned (%)
18.3
Duplicates removed (%)
80.3
Number of peaks
1886 (qval < 1E-05)

hg38

Number of total reads
29405113
Reads aligned (%)
26.4
Duplicates removed (%)
72.5
Number of peaks
2056 (qval < 1E-05)

Base call quality data from DBCLS SRA