Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Pro-B cells
NA
NA

Attributes by original data submitter

Sample

source_name
Pax5+/- ProB, no treatment, input
strain/background
C57BL/6J
genotype/variation
Pax5+/-
cell type
ProB
treatment
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-Seq: Chromatin immunoprecipitation using anti-Ebf1 antibody (Vendor: Millipore, cat# ABE1294, lot# Q2399134). ChIP-Seq: Samples were sent to UCLA Clinical Microarray Core for library preparation. Covaris M220 was used to shear ChIP-DNA. The library was generated using Nugen Ovation Ultra Low DR kit following standard procedures. Libraries were run on the Illumina HiSeq 2500.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
80271122
Reads aligned (%)
98.7
Duplicates removed (%)
15.9
Number of peaks
607 (qval < 1E-05)

mm9

Number of total reads
80271122
Reads aligned (%)
98.4
Duplicates removed (%)
15.8
Number of peaks
684 (qval < 1E-05)

Base call quality data from DBCLS SRA