GSM1695667: Ebf1 Chip-seq Ebf1+/- ProB no treatment; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Ebf1
Cell type
Cell type Class
Blood
Cell type
Pro-B cells
NA
NA
Attributes by original data submitter
Sample
source_name
Ebf1+/- ProB, no treatment, Ebf1 ChIP
strain/background
C57BL/6J
genotype/variation
Ebf1+/-
cell type
ProB
treatment
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-Seq: Chromatin immunoprecipitation using anti-Ebf1 antibody (Vendor: Millipore, cat# ABE1294, lot# Q2399134). ChIP-Seq: Samples were sent to UCLA Clinical Microarray Core for library preparation. Covaris M220 was used to shear ChIP-DNA. The library was generated using Nugen Ovation Ultra Low DR kit following standard procedures. Libraries were run on the Illumina HiSeq 2500.