Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Kmt2d

Cell type

Cell type Class
Blood
Cell type
Naive T cells
NA
NA

Attributes by original data submitter

Sample

source_name
Naive CD4 T cells
strain
C57BL/6 x 129
tissue
lymph nodes
treatment
wild type
chip antibody
MLL4 (PMCID: PMC3869375)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For RNA-Seq: the total RNAs were purified using Qiagen's miRNeasy micro kit (Qiagen cat# 217084) plus Qiagen's DNase set (Qiagen cat# 79254) for on-column DNase digestion option. PolyA-tailed RNAs were purified from pure total RNA using Dynabeads® mRNA DIRECT™ Kit (ambion life technologies cat# 61012). ChIP and Hi-C follow the protocols described previously in (PMID: 17512414) and (PMID: 25497547 ) with minor modifications. Library constructions for ChIP-Seq, RNA-Seq and Hi-C follow the protocols described previously in (PMID: 17512414), (PMID: 19528076) and (PMID: 25497547 ) with minor modifications.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
23177136
Reads aligned (%)
86.1
Duplicates removed (%)
42.7
Number of peaks
5194 (qval < 1E-05)

mm9

Number of total reads
23177136
Reads aligned (%)
86.0
Duplicates removed (%)
42.8
Number of peaks
5183 (qval < 1E-05)

Base call quality data from DBCLS SRA