Antigen

Antigen Class

TFs and others

Antigen

NPAT

Cell type

Cell type Class

Neural

Cell type

hTERT RPE-1

Primary Tissue

Eye

Tissue Diagnosis

Normal

Sample

source_name

hTERT-RPE1

cell line

hTERT-RPE1

cell type

Epithelial cells immortalized with hTERT

chip antibody

NPAT (sc-67007, Santa Cruz Biotechnology)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were cross-linked by 1% formaldehyde, and DNA was sonicated to 200- 400 bp fragments. Antibodies (5 µg) were added and collected using protein G Sepharose (GE). Cross-links were reversed and proteins digested by incubation with proteinase K at 65 °C overnight. DNA was then purified using Qiagen PCR purification kit. Libraries were constructed according to Illuminas standard protocol.

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

hg38

Number of total reads

35903342

Reads aligned (%)

75.2

Duplicates removed (%)

23.2

Number of peaks

862 (qval < 1E-05)

hg19

Number of total reads

35903342

Reads aligned (%)

74.5

Duplicates removed (%)

24.6

Number of peaks

1133 (qval < 1E-05)