GSM1693021: SUM159 ChIPSeq H3K27Ac; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Breast
Cell type
SUM 159PT
Primary Tissue
Breast
Tissue Diagnosis
Anaplastic Carcinoma
Attributes by original data submitter
Sample
source_name
Breast cancer cells
chip-antibody
H3K27Ac (Abcam, ab4729)
cell type
Basal-like cells
cell line
SUM159PT
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated using different antibodies. ChIPseq libraries were prepared by following Rubicon Thruplex-FD kit. Amplified fragments between 150 and 300bp (representing shear fragments between 50 and 200nt in length and ~100bp of primer sequence) were isolated by PAGE gel electrophoresis and purified.