Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GATA2

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
LNCaP shGATA2 R1881 (10nM,16h) GATA2 ChIP m76
cell line
LNCaP
cell type
prostate tumor
treatment
R1881 (10nM,16h)
antibody
GATA2 (santa cruz, catalog# sc-9008, lot# H1511)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model
Illumina HiScanSQ

hg19

Number of total reads
16193154
Reads aligned (%)
64.3
Duplicates removed (%)
15.9
Number of peaks
7637 (qval < 1E-05)

hg38

Number of total reads
16193154
Reads aligned (%)
65.5
Duplicates removed (%)
14.9
Number of peaks
7524 (qval < 1E-05)

Base call quality data from DBCLS SRA