Antigen

Antigen Class

TFs and others

Antigen

twi

Cell type

Cell type Class

Embryo

Cell type

2-4h embryos

NA

NA

Sample

source_name

tl10b embryos

developmental stage

2-4 hr after egg deposition

genotype/variation

tl10b

chip antibody

Custom Covance Twi (aa 340-490)

barcode kit

NEB-next-ChIP

library kit

NEB-next-ChIP

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq was performed according to He et al. (2011) with whole cell extract (WCE) derived from 100 mg embryos per ChIP

Sequencing Platform

instrument_model

Illumina HiSeq 2500

dm3

Number of total reads

24170982

Reads aligned (%)

54.7

Duplicates removed (%)

39.8

Number of peaks

17340 (qval < 1E-05)

dm6

Number of total reads

24170982

Reads aligned (%)

50.6

Duplicates removed (%)

44.0

Number of peaks

15545 (qval < 1E-05)