Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
twi

Cell type

Cell type Class
Embryo
Cell type
2-4h embryos
NA
NA

Attributes by original data submitter

Sample

source_name
tl10b embryos
developmental stage
2-4 hr after egg deposition
genotype/variation
tl10b
chip antibody
Custom Covance Twi (aa 340-490)
barcode kit
NEB-next-ChIP
library kit
NEB-next-ChIP

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq was performed according to He et al. (2011) with whole cell extract (WCE) derived from 100 mg embryos per ChIP

Sequencing Platform

instrument_model
Illumina HiSeq 2500

dm6

Number of total reads
24170982
Reads aligned (%)
90.4
Duplicates removed (%)
44.0
Number of peaks
15545 (qval < 1E-05)

dm3

Number of total reads
24170982
Reads aligned (%)
90.9
Duplicates removed (%)
39.8
Number of peaks
17340 (qval < 1E-05)

Base call quality data from DBCLS SRA