Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Embryo

Cell type

2-4h embryos

NA

NA

Sample

source_name

tl10b embryos

developmental stage

2-4 hr after egg deposition

genotype/variation

tl10b

chip antibody

H3K27me3 (Active Motif Carlsbad, CA, catalog #39155)

barcode kit

NEB-next-ChIP

library kit

NEB-next-ChIP

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq was performed according to He et al. (2011) with whole cell extract (WCE) derived from 100 mg embryos per ChIP

Sequencing Platform

instrument_model

Illumina HiSeq 2500

dm6

Number of total reads

30131820

Reads aligned (%)

97.0

Duplicates removed (%)

20.0

Number of peaks

5905 (qval < 1E-05)

dm3

Number of total reads

30131820

Reads aligned (%)

97.5

Duplicates removed (%)

16.5

Number of peaks

6069 (qval < 1E-05)