Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Embryo
Cell type
2-4h embryos
NA
NA

Attributes by original data submitter

Sample

source_name
gd7 embryos
developmental stage
2-4 hr after egg deposition
genotype/variation
gd7
chip antibody
H3K27ac (Active Motif Carlsbad, CA, catalog #39133)
barcode kit
NEB-next-ChIP
library kit
NEB-next-ChIP

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq was performed according to He et al. (2011) with whole cell extract (WCE) derived from 100 mg embryos per ChIP

Sequencing Platform

instrument_model
Illumina HiSeq 2500

dm3

Number of total reads
24558754
Reads aligned (%)
97.2
Duplicates removed (%)
16.8
Number of peaks
9423 (qval < 1E-05)

dm6

Number of total reads
24558754
Reads aligned (%)
97.0
Duplicates removed (%)
19.4
Number of peaks
9705 (qval < 1E-05)

Base call quality data from DBCLS SRA