Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Embryo

Cell type

2-4h embryos

NA

NA

Sample

source_name

gd7 embryos

developmental stage

2-4 hr after egg deposition

genotype/variation

gd7

chip antibody

H3K27ac (Active Motif Carlsbad, CA, catalog #39133)

barcode kit

none

library kit

NEB-next-DNA

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq was performed according to He et al. (2011) with whole cell extract (WCE) derived from 100 mg embryos per ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

dm6

Number of total reads

39221792

Reads aligned (%)

92.7

Duplicates removed (%)

57.2

Number of peaks

11192 (qval < 1E-05)

dm3

Number of total reads

39221792

Reads aligned (%)

92.9

Duplicates removed (%)

54.8

Number of peaks

11574 (qval < 1E-05)