Antigen

Antigen Class

TFs and others

Antigen

Tal1

Cell type

Cell type Class

Blood

Cell type

G1E-ER4+E2

Tissue

Blood

Lineage

cellLine

Description

Gata1 restored erythroid cells, differentiation induced by estradiol (E2)

Sample

source_name

G1E-ER4

chip antibody

TAL1 (sc12984)

cell type

G1E-ER4

cell description

Gata1 restored erythroid cells, differentiation induced by estradiol (E2)

strain

129

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and GATA1(or TAL1)-DNA complexes were isolated with antibody. ChIP-exo libraries were prepared for sequencing using standard SOLiD & Illumina protocols with a minor modification; Reference paper:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813302/

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

12996249

Reads aligned (%)

80.7

Duplicates removed (%)

83.7

Number of peaks

860 (qval < 1E-05)

mm9

Number of total reads

12996249

Reads aligned (%)

80.5

Duplicates removed (%)

83.9

Number of peaks

862 (qval < 1E-05)