Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MYC

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF-7
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
mammary gland, adenocarcinoma. (PMID: 4357757), newly promoted to tier 2: not in 2011 analysis
antibody antibodydescription
Rabbit polyclonal to amino acids 1-262 of c-Myc human origin. Antibody Target: MYC
antibody targetdescription
transcription factor; c-Myc-encoded proteins function in cell proliferation,differentiation and neoplastic disease
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-764
controlid
wgEncodeEH000589
labversion
baseAlignCounts.pl v 1,fseq v 1.84, iff_MCF7,p-value cutoff: 0.05
replicate
1,2
treatment
estrogen
treatment description
45 min with 100 nM Estradiol (Crawford)
antibody
c-Myc
antibody antibodydescription
rabbit polyclonal to amino acids 1-262 of c-Myc human origin
antibody targetdescription
transcription factor; c-Myc-encoded proteins function in cell proliferation,differentiation and neoplastic disease
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-764
controlid
MCF-7/Input
labversion
align_on_cluster_bwa.pl v 1
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM822304: UT-A_ChipSeq_MCF-7_estrogen_c-Myc
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
118116975
Reads aligned (%)
84.8
Duplicates removed (%)
12.7
Number of peaks
39756 (qval < 1E-05)

hg38

Number of total reads
118116975
Reads aligned (%)
86.3
Duplicates removed (%)
11.6
Number of peaks
36652 (qval < 1E-05)

Base call quality data from DBCLS SRA