GSM1684572: PANC1 CTCF CAGATC; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CTCF
Cell type
Cell type Class
Pancreas
Cell type
PANC-1
Tissue
Pancreas/Duct
Disease
Epithelioid Carcinoma
Attributes by original data submitter
Sample
source_name
PANC1 Cancer Cell line
cell line
PANC1
antibody
anti-CTCF
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq library preparation is described in O’Geen et al (Methods Mol Biol. 2011;791:265-286) using protein A/G beads to collect the immunoprecipitates. Approximately 5 x 10^7 MCF7 and PANC1 cells were crosslinked with 1% formaldehyde for 10 minutes at room temperature, crosslinking was quenched with 0.125 M glycine for 5 minutes at room temperature and cell pellets were collected and stored at -80°C. Nuclei were digested with 2000U HindIII prior to dilute solid-surface ligation reactions. For the ChIP-Seq, the anti-CTCF antibody was from Cell Signaling Technology CTCF (D31H2) XP® Lot 1, 3418S.