Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AGO2

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
chronic myelogenous leukemia cell line
cell line
K562
antibody
AGO2

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Formaldehyde cross-linked nuclei were sonicated and AGO2 was purified using indicated antibody and protein A beads. Immunopurified DNA was reverse-cross-linked, phenol:chloroform extracted and ethanol precipitated Library was constructed using Illuina Tru-seq branched adapters and subjected to 25 PCR cycles

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg19

Number of total reads
41741570
Reads aligned (%)
6.0
Duplicates removed (%)
82.1
Number of peaks
1374 (qval < 1E-05)

hg38

Number of total reads
41741570
Reads aligned (%)
6.4
Duplicates removed (%)
81.0
Number of peaks
1406 (qval < 1E-05)

Base call quality data from DBCLS SRA