GSM1682261: AGO2 ChIP-seq Millipore 04-642 rep 1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
AGO2
Cell type
Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous
Attributes by original data submitter
Sample
source_name
chronic myelogenous leukemia cell line
cell line
K562
antibody
AGO2
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Formaldehyde cross-linked nuclei were sonicated and AGO2 was purified using indicated antibody and protein A beads. Immunopurified DNA was reverse-cross-linked, phenol:chloroform extracted and ethanol precipitated Library was constructed using Illuina Tru-seq branched adapters and subjected to 25 PCR cycles