Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Kmt2a

Cell type

Cell type Class
Blood
Cell type
Bone Marrow Cells
MeSH Description
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.

Attributes by original data submitter

Sample

source_name
Bone marrow
cell type
MLL-AF9 transduced mouse bone marrow cells
agent
DMSO
antibody
MLL (anti-rabbit antibody made by our lab)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP experiments were performed using 1E8 cells and 15µl of antibodies per ChIP according to standard protocols. Libraries were prepared with the Illumina TruSeq protocol according to the manufacturer’s protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
69104506
Reads aligned (%)
25.3
Duplicates removed (%)
52.5
Number of peaks
354 (qval < 1E-05)

mm9

Number of total reads
69104506
Reads aligned (%)
25.2
Duplicates removed (%)
52.7
Number of peaks
369 (qval < 1E-05)

Base call quality data from DBCLS SRA