Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Gonad
Cell type
Testis
MeSH Description
The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.

Attributes by original data submitter

Sample

source_name
Mouse_Testis
strain
FVB
tissue
Testis pooled
age
84 days post partum
genotype
Trim33 +/+
chip antibody
input

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Testis were removed and pooled from 3 mice and flash frozen on dry ice. Testis were sent to Active Motif (CA, USA) who then carried out ChIP and library construction as well as Input DNA library construction (Cat No. 25001 and 25046). ChIP libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
40791103
Reads aligned (%)
90.8
Duplicates removed (%)
9.2
Number of peaks
1230 (qval < 1E-05)

mm9

Number of total reads
40791103
Reads aligned (%)
90.7
Duplicates removed (%)
9.2
Number of peaks
1196 (qval < 1E-05)

Base call quality data from DBCLS SRA