Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me1

Cell type

Cell type Class
Pancreas
Cell type
PANC-1
Tissue
Pancreas/Duct
Disease
Epithelioid Carcinoma

Attributes by original data submitter

Sample

source_name
PANC-1
biomaterial_provider
ATCC
biomaterial_type
immortalized cell line
lab
USC
lab description
Farnham - University of Southern California
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
line
PANC-1
cell organism
human
cell description
pancreatic carcinoma, (PMID: 1140870) PANC-1 was established from a pancreatic carcinoma, which was extracted via pancreatico-duodenectomy specimen from a 56-year-old Caucasian individual. Malignancy of this cell line was verified via in vitro and in vivo assays.
cell karyotype
cancer
cell lineage
endoderm
cell sex
M
treatment
None
treatment description
No special treatment or protocol applies
antibody
H3K4me1_(pAb-037-050)
antibody antibodydescription
Polyclonal antibody raised in rabbit against histone H3 containing the monomethylated lysine 4 (H3K4me1), using a KLH-conjugated synthetic peptide. Antibody Target: H3K4me1
antibody targetdescription
Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution.Histone tails undergo numerous posttranslational modifications. This antibodies recognizes histone H3 monomethylated on lysine 4.
antibody vendorname
Diagenode
antibody vendorid
pAb-037-050
control
UCDavis
control description
Input library was prepared at UC Davis.
control
UCDavis
control description
Input library was prepared at UC Davis.
controlid
wgEncodeEH002070
replicate
1

Sequenced DNA Library

library_name
GSM818827: USC_ChipSeq_PANC-1_H3K4me1_(pAb-037-050)_UCDavis
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

hg38

Number of total reads
57410048
Reads aligned (%)
94.0
Duplicates removed (%)
18.0
Number of peaks
3289 (qval < 1E-05)

hg19

Number of total reads
57410048
Reads aligned (%)
93.4
Duplicates removed (%)
19.1
Number of peaks
3076 (qval < 1E-05)

Base call quality data from DBCLS SRA