GSM1669133: JC2565 Xenograft MCF7 ER Vehicle r2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ESR1
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
Xenograft_MCF7_ER_Vehicle
implated cell line/type
MCF7; MCF7-Luc2/YFP cells
host strain
age-matched female NOD/SCID/IL2Rg-/- (NSG)
tissue
xenografts tumors in vivo
treatment groupd
Vehicle
chip antibody
ERa Antibody (HC-20)
chip antibody vendor
Santa Cruz
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
immunoprecipitated DNA was end-repaired, A-tailed, ligated to the sequencing adapters, amplified by 18 cycles of PCR and size selected (200-300 bp) followed by single end 4 sequencing on an Illumina HiSeq 2000 according to the manufacturer’s recommendationas described in Chandra, T. et al. Independence of Repressive Histone Marks and ChromatinCompaction during Senescent Heterochromatic Layer Formation. Mol Cell 47, 203–214 (2012)