Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
Xenograft_MCF7_ER_Vehicle
implated cell line/type
MCF7; MCF7-Luc2/YFP cells
host strain
age-matched female NOD/SCID/IL2Rg-/- (NSG)
tissue
xenografts tumors in vivo
treatment groupd
Vehicle
chip antibody
ERa Antibody (HC-20)
chip antibody vendor
Santa Cruz

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
immunoprecipitated DNA was end-repaired, A-tailed, ligated to the sequencing adapters, amplified by 18 cycles of PCR and size selected (200-300 bp) followed by single end 4 sequencing on an Illumina HiSeq 2000 according to the manufacturer’s recommendationas described in Chandra, T. et al. Independence of Repressive Histone Marks and ChromatinCompaction during Senescent Heterochromatic Layer Formation. Mol Cell 47, 203–214 (2012)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
16996031
Reads aligned (%)
81.0
Duplicates removed (%)
4.2
Number of peaks
785 (qval < 1E-05)

hg38

Number of total reads
16996031
Reads aligned (%)
83.0
Duplicates removed (%)
2.7
Number of peaks
620 (qval < 1E-05)

Base call quality data from DBCLS SRA