Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF7_ER_3hr_E2
cell line
MCF7
cell type
ER+ breast cancer cell line
condition
E2
chip antibody
ERa Antibody (HC-20)
chip antibody vendor
Santa Cruz

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Immunoprecipitated DNA was end-repaired, A-tailed, ligated to the sequencing adapters, amplified by 18 cycles of PCR and size selected (200-300 bp) followed by single end 50bp sequencing on an Illumina HiSeq 2000 according to the manufacturer’s recommendations

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
21596866
Reads aligned (%)
83.1
Duplicates removed (%)
10.4
Number of peaks
3518 (qval < 1E-05)

hg38

Number of total reads
21596866
Reads aligned (%)
85.9
Duplicates removed (%)
8.3
Number of peaks
3520 (qval < 1E-05)

Base call quality data from DBCLS SRA