Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EP300

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF7_p300_R5020_3hr
cell line
MCF7
cell type
ER+ breast cancer cell line
condition
R5020
chip antibody
p300 Antibody (C-20)
chip antibody vendor
Santa Cruz

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Immunoprecipitated DNA was end-repaired, A-tailed, ligated to the sequencing adapters, amplified by 18 cycles of PCR and size selected (200-300 bp) followed by single end 50bp sequencing on an Illumina HiSeq 2000 according to the manufacturer’s recommendations

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
14737825
Reads aligned (%)
13.5
Duplicates removed (%)
83.4
Number of peaks
1033 (qval < 1E-05)

hg38

Number of total reads
14737825
Reads aligned (%)
14.4
Duplicates removed (%)
82.3
Number of peaks
1025 (qval < 1E-05)

Base call quality data from DBCLS SRA