Antigen

Antigen Class

TFs and others

Antigen

ESR1

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MCF7_ER_Full_Media_3hr

cell line

MCF7

cell type

ER+ breast cancer cell line

condition

Full_Media

chip antibody

ERa Antibody (HC-20)

chip antibody vendor

Santa Cruz

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Immunoprecipitated DNA was end-repaired, A-tailed, ligated to the sequencing adapters, amplified by 18 cycles of PCR and size selected (200-300 bp) followed by single end 50bp sequencing on an Illumina HiSeq 2000 according to the manufacturer’s recommendations

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg19

Number of total reads

23051326

Reads aligned (%)

95.5

Duplicates removed (%)

9.2

Number of peaks

26725 (qval < 1E-05)

hg38

Number of total reads

23051326

Reads aligned (%)

96.0

Duplicates removed (%)

8.5

Number of peaks

26711 (qval < 1E-05)