Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EP300

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
T47D_p300_R5020_3hr
cell line
T47D
cell type
ER+ breast cancer cell line
condition
R5020
chip antibody
p300 Antibody (C-20)
chip antibody vendor
Santa Cruz

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Immunoprecipitated DNA was end-repaired, A-tailed, ligated to the sequencing adapters, amplified by 18 cycles of PCR and size selected (200-300 bp) followed by single end 50bp sequencing on an Illumina HiSeq 2000 according to the manufacturer’s recommendations

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
29228781
Reads aligned (%)
92.7
Duplicates removed (%)
5.0
Number of peaks
13415 (qval < 1E-05)

hg38

Number of total reads
29228781
Reads aligned (%)
94.7
Duplicates removed (%)
3.8
Number of peaks
13392 (qval < 1E-05)

Base call quality data from DBCLS SRA