GSM1667157: HuCCT1 HAYAP5SA; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
TFs and others
Cell type Class
Attributes by original data submitter
HuCCT1 human cell line
Sequenced DNA Library
Cells were cross-linked in 1% formaldehyde for 10 minutes at room temperature after which the reaction was stopped by addition of 0.125M glycine. Cells were lysed and harvested in ChIP buffer (100 mM Tris at pH 8.6, 0.3% SDS, 1.7% Triton X-100 and 5 mM EDTA) and the chromatin disrupted by sonication using a Diagenode Bioruptor sonicator UCD-200 to obtain fragments of 200-500 bp in size. Suitable amounts of chromatin were incubated with specific antibodies overnight. Antibodies used are: YAP (Cell Signaling – Cat. # 14074), TEAD1 (BD – Cat. # 610922), TEAD4 (Abcam – Cat. # ab58310), TAZ (Cell signaling Cat. # 4883), HA (Covance – Cat. # MMS-101P) Libraries were generated using NEBNext Ultra DNA Library Prep Kit for Illumina and barcoded using NEBNext Multiplex Oligos for Illumina according to manufacturer recommendation.