Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Gonad

Cell type

Testis

MeSH Description

The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.

Sample

source_name

Decapsulated testis

strain

C57BL/6|129/Sv|FVB/N Mixed BKG

chip antibody

none, input

tissue

Testis

age

post natal day 29-30

genotype

wild type

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatine was isolated, fragmented by sonication and immunoprecipitated with anti Rfx2 antibody Libraries were prepared according to Illumina's instructions using the Truseq ChIP-seq protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

23086632

Reads aligned (%)

90.4

Duplicates removed (%)

17.5

Number of peaks

742 (qval < 1E-05)

mm9

Number of total reads

23086632

Reads aligned (%)

90.2

Duplicates removed (%)

17.6

Number of peaks

763 (qval < 1E-05)