GSM1666129: KM2 CD133 neg NANOG IP; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Nanog
Cell type
Cell type Class
Liver
Cell type
Liver tumor
NA
NA
Attributes by original data submitter
Sample
source_name
mouse Tumor intitating stem cells
strain
C57BL/6
cell type
Tumor inititating stem cells
cell marker
CD133-
tissue
liver tumor
chip antibody
Nanog, Abcam ab21624
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Four pairs of TICs and Nanog-/CD133-/CD49f+ control cells (~1x10^5 per mouse) were isolated from four independent mouse liver tumors. ChIP was performed with NANOG antibody using CD 133+ as well as CD133– cell lines following a standard protocol as suggested by manufacturer (Millipore). In parallel, isotype control antibody was used as a control. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit. Briefly, DNA was end-repaired and blunt, phosphorylated ends were treated with Klenow fragment and dATP to yield a protruding 3- 'A' base for ligation of Illumina's adapters. After adapter ligation DNA was PCR amplified with Illumina primers for 15 cycles and library fragments of ~250 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Illumina Hi-Seq following the manufacturer's protocols.sequences) were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.