Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EP300

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
H1-hESC
biomaterial_provider
WiCell Research Institute
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
Rabbit Polyclonal. Antibody Target: P300
antibody targetdescription
EP300(c-20)
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-585
controlid
SL1398
labexpid
SL2250,SL1883
replicate
1,2
softwareversion
MACS
antibody
p300
antibody antibodydescription
Rabbit Polyclonal
antibody targetdescription
EP300(c-20)
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-585
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.2
protocol description
2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
controlid
SL1398
labexpid
SL2250
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803542: HudsonAlpha_ChipSeq_H1-hESC_p300_v041610.2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
46380456
Reads aligned (%)
78.8
Duplicates removed (%)
10.1
Number of peaks
18482 (qval < 1E-05)

hg38

Number of total reads
46380456
Reads aligned (%)
80.5
Duplicates removed (%)
8.8
Number of peaks
18847 (qval < 1E-05)

Base call quality data from DBCLS SRA