Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
POU2F2

Cell type

Cell type Class
Blood
Cell type
GM12878
Tissue
blood
Lineage
mesoderm
Description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Attributes by original data submitter

Sample

source_name
GM12878
biomaterial_provider
GM12878
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus
antibody antibodydescription
Oct-2(C-20) is an affinity purified rabbit polyclonal antibody raised against a peptide mapping at the C-terminus of Oct-2 of human origin. Recommended for detection of Oct-2 of m, r and h origin by WB, IP, IF, IHC(P) and ELISA. Antibody Target: POU2F2
antibody targetdescription
Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-233
protocol description
one 15-cycle round of PCR (Myers)
controlid
SL1395,SL516
labexpid
SL648,SL851,SL614
labversion
SL648,SL614
replicate
1,3,2
softwareversion
MACS
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-233
treatment
None
treatment description
No special treatment or protocol applies
protocol
PCR1x
protocol description
1-cycle of PCR (Myers)
controlid
SL516
labexpid
SL614
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803534: HudsonAlpha_ChipSeq_GM12878_POU2F2_PCR1x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
130520136
Reads aligned (%)
66.1
Duplicates removed (%)
11.5
Number of peaks
44228 (qval < 1E-05)

hg38

Number of total reads
130520136
Reads aligned (%)
67.5
Duplicates removed (%)
10.8
Number of peaks
43077 (qval < 1E-05)

Base call quality data from DBCLS SRA