Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SPI1

Cell type

Cell type Class
Blood
Cell type
GM12878
Tissue
blood
Lineage
mesoderm
Description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Attributes by original data submitter

Sample

source_name
GM12878
biomaterial_provider
GM12878
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus
antibody antibodydescription
Rabbit Polyclonal IgG. Antibody Target: PU1
antibody targetdescription
PU.1 (H-135)
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-22805
protocol description
one 15-cycle round of PCR (Myers)
controlid
SL1394,SL516
labexpid
SL612,SL963,SL649
labversion
SL649
replicate
1,3,2
softwareversion
MACS
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-22805
treatment
None
treatment description
No special treatment or protocol applies
protocol
PCR1x
protocol description
1-cycle of PCR (Myers)
controlid
SL516
labexpid
SL612
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803531: HudsonAlpha_ChipSeq_GM12878_PU.1_PCR1x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
210191297
Reads aligned (%)
23.5
Duplicates removed (%)
41.4
Number of peaks
60982 (qval < 1E-05)

hg38

Number of total reads
210191297
Reads aligned (%)
24.1
Duplicates removed (%)
40.4
Number of peaks
58504 (qval < 1E-05)

Base call quality data from DBCLS SRA