Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MAX

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
Rabbit polyclonal IgG, epitope mapping at the C-terminus of Max of human origin. Antibody Target: Max
antibody targetdescription
The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Multiple alternatively spliced transcript variants have been described for this gene but the full-length nature for some of them is unknown (RefSeq).
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-197
controlid
SL1396,SL1397
labexpid
SL2945,SL3070
replicate
1,2
cell sex
F
antibody
Max
antibody antibodydescription
Rabbit polyclonal IgG, epitope mapping at the C-terminus of Max of human origin.
antibody targetdescription
The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Multiple alternatively spliced transcript variants have been described for this gene but the full-length nature for some of them is unknown (RefSeq).
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-197
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.2
protocol description
2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
controlid
SL1396
labexpid
SL2945
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803523: HudsonAlpha_ChipSeq_K562_Max_v041610.2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
68077778
Reads aligned (%)
69.0
Duplicates removed (%)
19.9
Number of peaks
80803 (qval < 1E-05)

hg38

Number of total reads
68077778
Reads aligned (%)
70.0
Duplicates removed (%)
19.1
Number of peaks
81056 (qval < 1E-05)

Base call quality data from DBCLS SRA