Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EP300

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
T-47D
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
epithelial cell line derived from a mammary ductal carcinoma.
antibody antibodydescription
Rabbit Polyclonal. Antibody Target: P300
antibody targetdescription
EP300(c-20)
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-585
treatment description
1 h with 0.02% Dimethyl sufloxide (DMSO) (Myers)
controlid
SL1881
labexpid
SL3015,SL2633
replicate
1,2
softwareversion
MACS
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-585
treatment
DMSO_0.02pct
treatment description
1 h with 0.02% DMSO (Myers)
protocol
v041610.2
protocol description
2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
controlid
SL1881
labexpid
SL2633
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803522: HudsonAlpha_ChipSeq_T-47D_p300_DMSO_0.02pct_v041610.2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
50681479
Reads aligned (%)
81.0
Duplicates removed (%)
8.2
Number of peaks
17831 (qval < 1E-05)

hg38

Number of total reads
50681479
Reads aligned (%)
83.0
Duplicates removed (%)
6.8
Number of peaks
17703 (qval < 1E-05)

Base call quality data from DBCLS SRA