Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SRF

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
Rabbit polyclonal. Antibody Target: SRF
antibody targetdescription
Serum response transcription factor
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-335
controlid
SL2455
labexpid
SL3272,SL3162
replicate
1,2
softwareversion
MACS
antibody
SRF
antibody antibodydescription
Rabbit polyclonal
antibody targetdescription
Serum response transcription factor
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-335
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.1
protocol description
2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)
controlid
SL2455
labexpid
SL3162
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803520: HudsonAlpha_ChipSeq_K562_SRF_v041610.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
49386836
Reads aligned (%)
63.5
Duplicates removed (%)
25.9
Number of peaks
5929 (qval < 1E-05)

hg38

Number of total reads
49386836
Reads aligned (%)
64.7
Duplicates removed (%)
24.9
Number of peaks
5790 (qval < 1E-05)

Base call quality data from DBCLS SRA