Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
T-47D
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
epithelial cell line derived from a mammary ductal carcinoma.
antibody antibodydescription
Rabbit affinity purified polyclonal antibody. Epitope is the C-terminus of human estrogen receptor alpha (ESR1). Recommended for detection of estrogen receptor of m, r and h origin by WB, IP, IF, IHC(P) and ELISA. Antibody Target: ESR1
antibody targetdescription
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5-prime UTRs and use different promoters. (provided by RefSeq)
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-543
treatment description
1 h with 0.02% Dimethyl sufloxide (DMSO) (Myers)
controlid
SL1216,SL1490
labexpid
SL1216,SL1490
replicate
1,2
antibody targetdescription
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5-prime UTRs and use different promoters. (provided by RefSeq)
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-543
treatment
DMSO_0.02pct
treatment description
1 h with 0.02% DMSO (Myers)
protocol
v041610.2
protocol description
2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
controlid
SL1216
labexpid
SL1216
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803519: HudsonAlpha_ChipSeq_T-47D_ERalpha_a_DMSO_0.02pct_v041610.2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
41707848
Reads aligned (%)
72.8
Duplicates removed (%)
8.6
Number of peaks
2705 (qval < 1E-05)

hg38

Number of total reads
41707848
Reads aligned (%)
74.8
Duplicates removed (%)
7.0
Number of peaks
3476 (qval < 1E-05)

Base call quality data from DBCLS SRA