Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BCL3

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
lab
HudsonAlpha
lab description
Myers - Hudson Alpha Institute for Biotechnology
datatype
ChipSeq
datatype description
ChIP-Seq
cell
K562
cell organism
human
cell description
leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell karyotype
cancer
cell lineage
mesoderm
cell sex
F
antibody
BCL3
antibody antibodydescription
Bcl-3(C-14) is an affinity purified rabbit polyclonal antibody raised against a peptide mapping at the C-terminus of Bcl-3 of human origin. Recommended for detection of Bcl-3 of m, r and h origin by WB, IP, IF and ELISA.
antibody targetdescription
This gene is a proto-oncogene candidate. It is identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. The protein encoded by this gene contains seven ankyrin repeats, which are most closely related to those found in I kappa B proteins. This protein functions as a transcriptional co-activator that activates through its association with NF-kappa B homodimers. The expression of this gene can be induced by NF-kappa B, which forms a part of the autoregulatory loop that controls the nuclear residence of p50 NF-kappa B. (Provided by RefSeq).
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-185
treatment
None
treatment description
No special treatment or protocol applies
protocol
PCR1x
protocol description
1-cycle of PCR (Myers)
controlid
SL2455
labexpid
SL1196
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803518: HudsonAlpha_ChipSeq_K562_BCL3_PCR1x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
39078535
Reads aligned (%)
68.2
Duplicates removed (%)
20.8
Number of peaks
1307 (qval < 1E-05)

hg38

Number of total reads
39078535
Reads aligned (%)
69.1
Duplicates removed (%)
20.0
Number of peaks
1244 (qval < 1E-05)

Base call quality data from DBCLS SRA